11 Enhancement of Food Production - part 03 - Tissue culture
11 Enhancement of Food Production - part 03 - Tissue culture Tissue culture : Is actually a collection of different techniques. T echnique of plant biotechnology. Here, isolated cells, tissues, organs are grown ‘in vitro’ on a solid/ liquid nutrient medium, under aseptic and controlled conditions of light, humidity and tempreature, for achieving different objectives. Part of plant used in tissue culture is called explant . Plant tissue culture is based on principle of Totipotency which is an inherent ability of living plant cell to grow, divide, redivide and give rise to a whole plant. Haberlandt (1902 for the first time conceived this idea and developed the concept of in vitro cell culture ( plant morphogenesis ). Medium contains all essential minerals, sources for carbohydrates, proteins and fats, water, growth hormones, vitamins and agar (for callus culture). Most commonly preferred medium for tissue culture is MS (Murashige and Skoog) medium. Based on the nature of ex