5. Cell Structure And Organization - part 02 - Prokaryotic cells
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5. Cell Structure And Organization - part 02 - Prokaryotic cells
Kinds of cells :
- Living organisms are grouped into two main categories -
- Prokaryotes and
- Eukaryotes.
- The prokaryotes have simple cellular organization while eukaryotes exhibit high degree of organization.
A. Prokaryotic cells :
- It has chemically complex protective cell envelop.
- It does not have well-defined nucleus and other membrane bound cell organelles.
- Cell envelop is a three-layered structure with-
- outer glycocalyx
- middle cell wall and
- inner plasma membrane.
- Glycocalyx is present as either slime layer (loose sheath) or capsule (tough).
- Bacteria are better observed when stained.
- The most followed staining method is ‘Gram staining’ developed by Danish bacteriologist Hans Christian Gram.
- The cell wall is made up of peptidoglycan (in Gram positive bacteria) and murein (in Gram negative bacteria). It gives mechanical strength to the cell.
- Cell membrane is a phospholipid bilayer. All these structures give protection to the cell and also help in inter-cellular transport.
- In motile bacteria either cilia or flagella are found. Both are driven by rotatory movement produced by basal body (which works as motor).
- Other parts are filament and hook.
- Some other surface projections are the tubular pili (which help in inter-cellular communication) and fimbriae (for clinging to support).
- The cell membrane shows infoldings called mesosomes, which help in cell wall formation and DNA replication.
- Some bacteria especially photosynthetic cyanobacteria show more longer extensions called chromatophores.They carry photosynthetic pigments.
- The cytoplasm contains dense particles called ribosomes helping in protein synthesis.
- Ribosomes are described by their sedimentation rate in Svedberg units.
- Bacterial ribosome are 70S (composed of a larger subunit 50S + smaller subunit 30S).
- The term cell was first used by Robert Hooke (1665) in his book ‘‘Micrographic’’.
- Purkinje and Mohl (1835-37) discovered protoplasm.
- Camillo Golgi (1838) discovered the Golgi apparatus.
- Robert Brown (1881) discovered the Nucleus.
- Balbiani (1881) discovered chromosomes in salivary glands of Chironomus larva.
- Flemming (1882) studied cell division in detail and coined the term Mitosis.
- Porter (1945) discovered Endoplasmic Reticulum.
- C.Benda gave the name Mitochondria.
- C. de Duve (1955) discovered Lysosomes
Always remember :
- Genetic material in bacterium is a single chromosome made up of circular and coiled DNA. It remains attached to mesosome.
- This DNA undergoes a very typical replication pattern called as theta model of replication.
- The DNA is not associated with histone proteins (as in eukaryotes) hence not referred to as chromatin.
- Besides chromosomal DNA many bacteria show plasmids which are small circular DNA molecules carrying few genes.
- They are termed as extrachromosomal selfreplicating DNA molecules.
- They are of two basic types
- F – plasmid for reproduction and
- R – plasmid for resistance against antibiotics.
- Cytoplasm of prokaryotes is a pool of all necessary materials like water, enzymes, elements, amino acids, etc.
- Some inclusion bodies in form of organic (cyanophycean starch and glycogen) and inorganic granules (phosphate and sulphur) are also found.
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